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90 thoughts on “DNA cloning and recombinant DNA | Biomolecules | MCAT | Khan Academy

  1. Please make a video about isothermal assembly methods (such as Gibson Assembly). These are the dominant methods being used currently.

  2. That's amazing, to think we can do this inside cells using biochemistry to get what we want! Keep them coming Sal, loving these new (micro)bio vids.

  3. I find it really amazing that we can do this. However, a part of me wonders about the ethical considerations. Also, another part of me wonders if this is part of the reason why we have diseases that are resistant to antibacterials, which make them much harder to treat. Considering that some of these diseases are both highly contagious AND deadly if not treated (as is the case with certain strands of Tuberculosis) this is not a good combination to have randomly floating around out there, such as we do now have.

  4. closer but still not able to explain completely….how will we all of sudden will introduce antibiotic resistance gene in vector…won't it too will take process of colony formation selection of transformant same as that of our gene of interest…so by your video concept before clearing give rise to another doubt

  5. I am forever grateful to you because you are the ONLY REASON why I will pass my exam. Thank you thank you thank you!!! <3

  6. this is amazing. i have tried so hard to understand this (i have watched other videos, read an excerpt from a textbook etc.) and now i finally get it. thank you khan academy

  7. So were intentionally making bacteria antibiotic resistant? Seems like if there is any more serious bacteria in the plate or initial sample thats not good…

  8. Suppose we link our gene of interest to BamH I. Then grow the bacteria in ampicillin medium to distinguish between the transformants and non transformants . The recombinant bacteria with our gene of interest will die, how will we be able to use the dead bacterium?

  9. why are plasmids used in recombinant dna technology must have the ability to duplicate indepedently from the host cell? thx in advandance

  10. great video, thanks. But i have a question: How can we know that the plasmid does contain the sequence of interest and not another sequence that happens to use the same restriction enzyme?

  11. Man, I haven't seen this stuff in years….almost decadeS. I used to do this in college ….and didn't remember specifics. This brought it all back. Thank you.

  12. Isn't the replication of the plasmid independent from the replication of the bacterial DNA ? i.e isn't the plasmid capable of auto-replication ?

  13. I was wondering the following, this video tells me that DNA is taken from one organism and put into another organism's plasmid so that that organism can go and create a desired protein. My question is then, does the bacteria know how to splice the introns and exons when it's creating the desired protein. Much more logical to me would be that they take ripe mRNA, (already spliced correctly) reverse transcript that and put it in the plasmid, since bacteria don't have introns and exons.

  14. Mutations are a big danger. Recombiant DNA vaccines may cause your cells to mutate. Oregano oil can kill virus and bacteria. And they cannot develop immunity like antibiotics. Why can't they use this oil some how internally when there is an outbreak. I use the oil when I have a sore throat. And oil knocks it right out.

  15. I live for Sal's mistakes in his videos; so funny. "…and this isn't fully understood how the sheet hock…" Waiting for the bloopers video.

  16. Thank you so much. I've got one question, how do you know the plasmid with the antibiotic resistant gene will take up the gene of interest and not the other plasmid without the antibiotic resistant gene?

  17. I have a question ,you wish to clone the gene for enhanced green fluorescent protein (eGFP) into
    pGEM3z that will be translated from 171bp to 1493bp. using the checklist below how one can draw
    a vector map with eGFP insert included.
    Include the following details:
    – Vector name
    – Insert size
    – Restriction enzyme (s) and location
    – Direction of replication
    – Selection agent(s)
    – Promoter region

    And with reference to the eGFP in your vector, describe the cloning strategy that proves
    that genes can be moved between species.

  18. The heat shock is happening through CaCl2, which is counteracting the electrostatic repulsion between plasmid DNA the bacterial cellular membrane ( liposaccharide layer), mostly E.Coli as it is most competent bacteriathan for these procedures.

  19. Wait uhmm. if you mix an antibiotic into the plasmic and get the bacteria to swallow it. won't the bacteria die? or some survives?

  20. Thank you! I was reading my textbook and lecture notes, but still couldn’t understand it. This video explained everything perfectly 👌

  21. How do you know which restriction enzymes to use? How did you find out the sequence at both ends of the gene in the first place?

  22. How do you know if the plasmids taken up by the bacteria also contained the gene of interest? Couldn't some of the bacteria take up plasmids that gave them antibiotic resistance but did not include the gene of interest?

  23. This translates well in microbiology but how does an antibiotic resistant ecoli plasmid translate to animal species? You go into insulin but how does that work? Is it because we have altered the plasmid DNA which would render any ecoli effects useless?

  24. I can't sure that you respond to me, but I have a question. Is there any checking process that we can check the plasmid has a gene that we want to clone between 'Paste process' and 'Inserting the plasmid to the e.coli' ?

  25. how do we know that the bacterial colony with the desired product only have the antibiotic resistance?

  26. Sir, you are my favourite teacher on the Khan Academy platform. Great job !!! You helped me a lot during my studies

  27. You are awesome really, very clear and good video, thank you so much, we will wait for more videos

  28. I'm 22. But can GOM work on whatever ages we are? or it only works for the embryo? haha.. as an example in my age 22, can I get blue eyes?

  29. ive struggled so much to understand this topic in my first language lol and now i tried to it in english and your video was so helpful (and pretty) thank yaa

  30. now only if we could get the church to get off the case of cloning, we have the technology to build cloned human body parts and save millions

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